ABSTRACT. This study aims to analyze the application of chemical fertilizers (NPK) to the production of rubber latex, to analyze the application of organic fertilizer SUPERNASA to the production of rubber latex and to analyze the comparison of the application of fertilizer SUPERNASA to the production of rubber latex. The application of NPK fertilizer to PB20 rubber resulted in an average total of rubber latex of 31.36 grams while IRR39 rubber produced an average total of rubber latex of 29.98 grams, Giving organic supernase fertilizer to PB20 rubber resulted in a total rubber sap of 29.34 grams while the IRR39 rubber produces rubber sap of 28.44 grams. The treatment without fertilizer application on PB20 rubber produced 23.78 grams of rubber latex, while the IRR39 rubber was 21.44 grams. The treatment with the addition of fertilizer resulted in a higher amount of rubber latex, and the PB20 type of rubber produced greater rubber latex than the IRR39 rubber type with or without treatment.