Green grass jelly (Premna serratifolia L.) is a vine or coiled plant originating from Southeast 

Asia. Green grass jelly cultivation in Indonesia is still relatively low due to the difficulty of 

cultivating grass jelly plants, especially in terms of plant propagation. In general, propagation 

is done by stem cuttings. Growth regulators (PGR) that is often used is synthetic PGR, one 

of which is Rootone F, but its use is less effective in terms of economy. it is necessary to 

substitute with PGR derived from natural materials or from the use of household and 

industrial wastes such as coconut water, rice washing water, and tofu waste water. The 

purpose of this study was to determine the effect of soaking natural PGR in coconut water, 

rice washing water, and tofu waste water and to find out the best natural PGR ingredients for 

the growth of green grass jelly plants. This study used an experimental design used was a 

one-factor Completely Randomized Design (CRD), which consisted of four treatments and 

was repeated five times so that twenty experimental units were obtained with 3 samples 

each, requiring 60 plants. P1=0.1 gL-1 Rootone F (positive control), P2=450 ml.L-1 young 

coconut water, P3=450 ml.L-1 rice washing water, P4=450 ml.L-1 tofu waste water.