Abstrak

Pemberian vaksin monovalen A. hydrophila adalah salah satu upaya dalam pencegahan dan pengendalian serangan penyakit Motile aeromonas septicemia (MAS). Penelitian ini bertujuan untuk mengetahui pengaruh vaksin monovalen terhadap relative percent survival, rerata waktu kematian serta mengetahui dosis optimal untuk vaksinasi. Vaksin monovalen dibuat dengan metode heat killed dan variasi dosis vaksin dilakukan dengan cara serial dilution 10⁹, 10⁸, 10⁷, 10⁶ sel/mL. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Parameter yang diamati meliputi relative percent survival, sintasan, mortalitas, rerata waktu kematian, gejala klinis eksternal dan internal, dan kualitas air. Hasil penelitian menunjukkan pemberian vaksin dengan variasi dosis yang berbeda berpengaruh nyata terhadap parameter seperti relative percent survival, sintasan, mortalitas dan rerata waktu kematian, serta memberikan perlindungan dengan cara meminimalisir gejala klinis eksternal dan internal pada saat dilakukan uji tantang. Kualitas air masih dalam kisaran normal untuk kehidupan dan vaksinasi ikan papuyu. Dosis vaksin monovalen A. hydrophila yang terbaik terdapat pada perlakuan A 10⁶ sel/mL.

Kata Kunci: vaksin, monovalen, dosis, Aeromonas hydrophila, Anabas testudineus

Abstract

The usage of monovalent vaccines from strain local A. hydrophila is one of the efforts to prevent and control Motile aeromonas septicemia (MAS) diseases. The aim of study was to determine the effect of monovalent vaccies on relative percent survival, mean time to death, and to determine the optimal dose for vaccination. Monovalent vaccines was made using the heat killed method and dose variations were carried out by serial dilution 10⁹, 10⁸, 10⁷, 10⁶ sel/mL. The study used a Completely Randomized Designed (CRD) with 5 treatments and 3 replications. Parameters observed included relative percent survival, survival rate, mortality rate, mean time to death, external and internal clinical symptomps and water quality. The results showed that the usage of monovalent vaccines with dose variations from A. hydrophila had a significant effect on the parameters like relative percent survival, survival rate, mortality, mean time to death, and provided protection by minimizing external and internal clinical symptomps in challenge trials. The water quality is still within the normal range for both life and climbing perch vaccination. The best dose of A. hydrophila vaccines in this study was treatment A 10⁶ sel/mL.

Keywords: vaccine, monovalent, dose, Aeromonas hydrophila, Anabas testudineus