Jasmine (Jasminum sambac) leaves wererecognized for multipurpose use, the scent of jasmine could reduce stress and depression/anxiety, it has antiseptic, antibacterial, antifungal and effective treatment for skin irritations. Acne were a inflammatory skin disorder caused by P.acnes that can appear in a variety of ways, from little blemishes to obvious cysts. Some acne treatment may differ, depending to the type of acne treatment that may use such as retinoid drugs, antibiotic and azelaic and salicyclic acid.  Acne patch use hydrocolloid-producing chemicals. Hydrogel patches can kill acne-causing bacteria, medicated acne patches were also known as hydrogel patches. This study was conducted to determine the total phenolic content it in Jasminum Sambac extract in acne patch preparations. The research method began with preparation of sample simplicia. Preparation of Jasmine leaf ethanol extract, preparation of three acne patch formulations with various concentration of 25%, 30%, 35%. Folin-ciocalteau phenol reagent was used to determine the phenolic substances present in the patch preparation and gallic acid as the standard was used.  The results showed that the average of total phenolic content of J. sambac extracts in acne patch preparation was 72.018mgGAE/patch in formula I; formula II has 74.951 mgGAE/patch and Formula III has 82.240 mgGAE/patch. The conclusion obtained in this study is that the higher extract in the patch the higher the concentration of the total phenolic content.