ABSTRACT

ANTIBACTERIAL ACTIVITY OF RANGOON CREEPER (Combretum Indicum) FLOWER EXTRACT AGAINST Staphylococcus aureus and Escherichia coli bacteria  (By Micaellah Joy Custodio; Advisor       :        Pratika Viogenta, Amelia Khairunnisa; 2023; 86 Pages)

Rangoon creeper (Combretum indicum) is a valuable medicinal plant in tropical Asia. C. indicum plants have 2 variations, namely the rounded type and the elongated type. Flowers and bark are known for their antioxidant, antibacterial, and cytotoxic activity. There have been a number of investigations on the antibacterial efficacies of C. indicum species against S. aureus and E. coli. Antibacterial testing used the agar well diffusion method with extract concentrations of 20%, 15%, 10%, and 5%. The positive control used was ciprofloxacin and the negative control used was 10% DMSO. The results of the study showed that the ethanol flower extract of the Rangoon creeper (Combretum indicum) round and elongated type has antibacterial activity against S. aureus and E. coli bacteria. At a concentration of 20% for the round type were able to inhibit a diameter zone against S. aureus at 22.3 mm, for the the elongated type at concentrations of 20%, 15%, and 10% it has 13.6 mm, 7.5 mm, and9 mm. While for the ethanol extract of the Rangoon creeper (Combretum indicum) rounded type against E.coli bacteria, 20%, 15%, and 10% were able to inhibit a diameter zone at 16.3 mm, 10.2 mm, and 8 mm Therefore it can be concluded that the ethanol extract of C. indicum flower has antibacterial activity that is bacteriostatic and has a broad spectrum.

Keywords          :  Combretum indicum., antibacterial, Staphylococcus aureus, Escherichia coli