Anthropogenic activities such as the disposal of industrial and domestic waste into the waters cause a decrease in water quality, as is the case in the riparian areaa of ??Martapura river, Banjar Regency. Of course, this should be watched out for considering that the presence of waste will have an impact on the quality of the environment and the organisms that live in the environment. Water spinach (Ipomoea aquatica Forsk.) is a type of plant that is commonly found in the waters of Banjar Regency. Water spinach was chosen as a bioindicator because its presence can reflect a part that can represent the ecosystem being monitored. This research was conducted to determine the source of waste contamination from anthropogenic activities, to determine a depiction of water quality in the riparian areas of ??the Martapura river, and to determine the anatomical and morphological differences of water spinach affected by waste contamination. The research stations were located in Tambak Anyar Ulu Village, Pekauman Ulu Village, and Keraton Village, Banjar Regency, based on the presence of the dominant waste source. The results showed that the waste contamination came from the anthropogenic activities of the community around the research station. The status of the water quality in the riparian areas of Martapura river showed a total score of -18, indicating that the status of the water quality in the riparian areas of ?? Martapura river is classified as Class C water (medium polluted), and from the results of the MANOVA analysis it is concluded that the differences in sources of contamination at each research station affected the anatomy of water spinach in the riparian waters of Martapura river.