Peatlands are developed for the cultivation of plantation crops such as oil palm. However, recently the focus on the development of peatlands for this activity has been so great, especially in relation to the emission of carbon dioxide CO₂ into the atmosphere during land clearing, burning of biomass and maintenance of oil palm. The potential for peatland to be used as agricultural land must pay attention to environmental aspects such as the level of CO₂ emissions to these changes. This study aims to determine the effect of wood charcoal and empty oil palm fruit bunches on CO₂ emissions and their relationship to C-Mik and C/N ratios on peatlands overgrown with oil palm. This study used a randomized block design. The factors studied were the distribution of wood charcoal and empty palm fruit bunches at 4 levels, namely: wood charcoal + empty palm fruit bunches 0 kg; wood charcoal 0.75 kg; empty palm fruit bunches 0.75 kg and empty palm fruit bunches 0.375 kg + wood charcoal 0.375 kg. Each treatment was repeated 3 times, so that 12 experimental units were obtained. The results showed that on day 0 and day 45 the administration of wood charcoal and empty palm oil bunches had no effect on CO₂ emissions_, but on day 15 the results showed that the treatment had an effect on CO_2. emissions_2, soil microorganisms (C-mic) and unstable C/N ratios increased or decreased when sampling on different days.value of CO₂ gas emissions  empty palm oil bunches.