ABSTRAK

PENGARUH EKSTRAK TANAMAN BAWANG DAYAK (Eleutherine palmifolia (L.) Merr.) PADA KULIT TIKUS (Rattus novergicus) YANG DIPAPAR SINAR ULTRAVIOLET IN VIVO

Ditinjau dari aktivitas enzim superoksida dismutase (SOD) dan kadar O2 superoksida

Laily Wahyu Atanta

Paparan sinar ultraviolet pada kulit menyebabkan  terjadinya proses oksidasi sehingga terbentuknya ROS (reactive oxygen species). ROS dapat dinetralisir oleh antioksidan, salah satunya adalah enzim superoksida dismutase (SOD). Tanaman bawang dayak (Eleutherine palmifolia (L.) Merr.) mengandung senyawa bioaktif yang berfungsi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh efek pemberian ekstrak tanaman bawang dayak terhadap aktivitas enzim superoksida dismutase (SOD) dan kadar O2 superoksida pada kulit tikus setelah diberi paparan sinar ultraviolet. Metode penelitian ini adalah true experimental dengan post-test only with controlled group design. Subjek penelitian adalah 30 ekor tikus jantan yang dikelompokkan dalam 4 kelompok yaitu, kelompok kontrol (P0), kelompok yang hanya diberi paparan sinar ultraviolet (P1), kelompok yang hanya diberi ekstrak tanaman bawang dayak (P2), dan kelompok yang diberi ekstrak dan diberi paparan sinar ultraviolet (P3). Variabel yang diamati adalah aktivitas enzim SOD dan kadar O2 superoksida yang diukur dengan metode Mirscha dan Fridovich di Laboratorium Biokimia-Biomolekuler FK UNLAM. Hasil penelitian didapatkan aktivitas enzim SOD meningkat dan kadar O2 superoksida menurun pada kelompok tikus yang diberi ekstrak tanaman bawang dayak. Kesimpulan penelitian ini ekstrak tanaman bawang dayak dapat meningkatkan aktivitas enzim SOD dan menurunkan kadar O2 superoksida pada kelompok tikus yang dipajan sinar ultraviolet.

Kata-kata kunci : Radiasi ultraviolet, reactive oxygen species, superoksida dismutase, superoksida, bawang dayak

ABSTRACT

THE EFFECT OF BAWANG DAYAK (Eleutherine palmifolia (L.) Merr.) EXTRACT ON THE SKIN OF RATS (Rattus novergicus) EXPOSED TO ULTRAVIOLET LIGHT IN VIVO

In terms of superoxide dismutase enzyme activity and O2 superoxide levels

Laily Wahyu Atanta

Ultraviolet light exposure to the skin causes the oxidation process to form ROS (reactive oxygen species). ROS can be neutralized by antioxidants, one of which is the superoxide dismutase (SOD) enzyme. Bawang Dayak plant (Eleutherine palmifolia (L.) Merr.) contain bioactive compounds that function as antioxidants. This study aims to determine the effect of Bawang Dayak plant extract on the activity of the superoxide dismutase (SOD) enzyme and O2 superoxide levels in the skin of rats after being exposed to ultraviolet light. This research method is true experimental with post-test only with controlled group design. The research subjects were 30 male rats grouped in 4 groups, namely, the control group (P0), the group who were only given ultraviolet light exposure (P1), the group that was only given Bawang Dayak plant extract (P2), and the group given extract and given ultraviolet light exposure (P3). The variables observed were SOD enzyme activity and O2 superoxide levels as measured by the Mirscha and Fridovich method at the FK UNLAM Biochemistry-Biomolecular Laboratory. The results showed that SOD enzyme activity increased and O2 superoxide levels decreased in the group of rats given onion dayak plant extract. The conclusion of this study is that onion dayak plant extract can increase SOD enzyme activity and reduce O2 superoxide levels in groups of rats exposed to ultraviolet light.

Keywords : Ultraviolet radiation, oxidative stress, reactive oxygen species, superoxide dismutase, superoxide, bawang dayak