ABSTRAK

Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan ekstrak bunga rosela (Hibiscus sabdariffa L.) dalam pengencer tris kuning telur terhadap motilitas dan viabilitas spermatozoatozoa kambing Peranakan Etawah (PE) yang dipreservasi pada suhu 5°C. Penelitian dilaksanakan di Laboratorium Produksi Ternak, Fakultas Pertanian Universitas Lambung Mangkurat Banjarbaru pada April–Juli 2025, menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan. Perlakuan terdiri atas P0 (kontrol tanpa ekstrak rosela), P1 (1% ekstrak rosela), P2 (3% ekstrak rosela), dan P3 (5% ekstrak rosela). Parameter yang diamati adalah motilitas dan viabilitas spermatozoatozoa setiap hari selama penyimpanan hingga motilitas turun di bawah 40%.

Hasil penelitian menunjukkan bahwa penambahan ekstrak bunga rosela berpengaruh nyata terhadap motilitas dan viabilitas spermatozoatozoa selama penyimpanan dingin. Perlakuan terbaik diperoleh pada konsentrasi 3% (P2), yang mampu mempertahankan motilitas dan viabilitas spermatozoatozoa di atas standar minimal hingga hari ke-6 penyimpanan. Motilitas spermatozoatozoa P2 pada hari ke-6 masih mencapai 40%, sedangkan viabilitas mencapai 49,25%. Kandungan antioksidan pada ekstrak rosela diduga berperan melindungi spermatozoatozoa dari kerusakan oksidatif selama masa penyimpanan.

Dapat disimpulkan bahwa penambahan 3% ekstrak bunga rosela dalam pengencer tris kuning telur merupakan konsentrasi paling optimal untuk mempertahankan kualitas spermatozoatozoa kambing PE selama penyimpanan pada suhu 5°C.

Kata Kunci: spermatozoatozoa, rosela, motilitas, viabilitas, kambing PE, suhu 50C.

ABSTRACT

This study aimed to evaluate the effect of adding roselle flower extract (Hibiscus sabdariffa L.) to a tris-egg yolk extender on the motility and viability of Peranakan Etawah (PE) goat spermatozoatozoa preserved at 5°C. The research was conducted at the Animal Production Laboratory, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru, from April to July 2025, using a Completely Randomized Design (CRD) with four treatments and four replications. The treatments consisted of P0 (control without roselle extract), P1 (1% roselle extract), P2 (3% roselle extract), and P3 (5% roselle extract). Parameters observed were spermatozoatozoa motility and viability, measured daily until motility dropped below 40%.

The results showed that the addition of roselle flower extract significantly affected spermatozoatozoa motility and viability during cold storage. The best result was obtained with 3% roselle extract (P2), which maintained sperm motility and viability above the minimum standard until the 6th day of storage. On day 6, motility in the P2 treatment remained at 40%, while viability was 49.25%. The antioxidant content of roselle extract is thought to protect spermatozoatozoa from oxidative damage during storage.

It can be concluded that the addition of 3% roselle flower extract to tris-egg yolk extender is the most optimal concentration for maintaining the quality of PE goat spermatozoatozoa stored at 5°C.

Keywords: spermatozoatozoa, roselle, motility, viability, PE goat, cold storage.