This study aims to determine the effect of interval training on increasing VO2Max in adolescent pencak silat athletes at the Nurhati College in HST Regency. With a population of 24 people and a sample of 10 adolescent athletes selected through purposive sampling technique, this study used an experimental approach with a One Group Pre-Test-Post Design. The Multistage Fitness Test (MFT) was the tool used to obtain data, applying quantitative methods for this study. The average VO2Max increased by an average of 3.16 mL/kg/minute from 36.95 mL/kg/minute in the pre-test to 40.11 mL/kg/minute in the post-test. Before and after interval training therapy, there was a significant difference, as indicated by the significance value of the paired sample t-test statistic which was less than 0.08. Therefore, interval training has been proven to be successful in increasing VO2Max in adolescent pencak silat athletes at the Nurhati College in HST Regency. The findings of this study can be a basis or reference in developing physical training programs, especially those aimed at increasing athlete endurance.