E-Learning is an electronic-based learning media that utilizes computers, mobile phones that can be connected to the internet network. The e-learning system developed by Lambung Mangkurat University in each semester always provides a questionnaire that aims to collect criticism and suggestions from each student, but in the questionnaire there is no assessment in terms of appearance, convenience, and efficiency. Therefore, testing of the system is needed which aims to assess the level of acceptance of the application. In this study, the User Acceptance Testing method was used as a method of testing the level of acceptance of students and female students of Lambung Mangkurat University as End-Users and aims to find out whether the e-learning application has fulfilled the aspects contained in User Acceptance Testing and how the level of acceptance from students of Lambung Mangkurat University towards e-learning applications. The results showed that this e-learning system produced an acceptance level of the display aspect of 75.36% which resulted in a good description. In the aspect of convenience, it produces 71.64% with a good description and in the aspect of efficiency produces 70.36% with a good description.